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目的研究芍甘附子汤(SGFZ)加味对胶原诱导性关节炎(CIA)大鼠滑膜内皮抑素(ES)、血管生成抑素(AS)蛋白表达水平的影响。方法健康雌性Wistar大鼠60只,分为正常组10只和造模组50只,采用牛Ⅱ型胶原与完全弗氏佐剂建立大鼠CIA模型。造模成功后分组,正常组、模型组给予等量蒸馏水灌胃,雷公藤多甙组按8.75 mg/(kg·d)灌胃,SGFZ加味小、中、大剂量组依次按每毫升药液含0.708、1.42、2.83 g生药灌胃,均为每日1次,连续给药28天。造模后第0、14、28、42、56天观察大鼠关节炎指数(AI),灌胃治疗结束后Western blot法检测ES、AS表达水平。结果与正常组比较,造模组AI评分显著升高(P<0.01);经药物治疗后,与模型组比较,雷公藤多甙组与SGFZ加味大、中剂量组AI评分降低(P<0.01)。与正常组比较,模型组ES、AS蛋白表达水平均下调(P<0.01);与模型组比较,雷公藤多甙组和SGFZ加味各剂量组ES蛋白表达水平均上调(P<0.01),SGFZ加味大、中剂量组AS蛋白表达水平上调(P<0.01)。结论 SGFZ加味可有效改善CIA大鼠关节肿胀程度,其作用机制可能与调节ES、AS蛋白表达水平有关。
Objective To investigate the effect of Shaoganfuzi Decoction (SGFZ) on the synovial endostatin (ES) and angiopoietin (AS) protein expression in synovial - induced arthritis (CIA) rats. Methods Sixty healthy female Wistar rats were divided into normal group (n = 10) and model group (n = 50). CIA model was established by using type Ⅱ collagen and complete Freund’s adjuvant. After successful modeling, the rats in the normal group and model group were given the same amount of distilled water for gavage. The tripterygium glycosides group was given gavage at 8.75 mg / (kg · d), and the small, Containing 0.708,1.42,2.83 g crude drug gavage, are daily 1, continuous administration of 28 days. The arthritis index (AI) was observed on the 0th, 14th, 28th, 42th and 56th day after the model establishment. The expression of ES and AS was detected by Western blot. Results Compared with the normal group, the AI scores of model group were significantly increased (P <0.01). After drug treatment, AI scores of tripterygium glycosides group and SGFZ plus large and medium dose groups decreased (P <0.01) ). Compared with the normal group, the levels of ES and AS protein in the model group were significantly decreased (P <0.01). Compared with the model group, the levels of ES protein in the tripterygium glycosides group and the SGFZ-flavored group were all increased (P <0.01) The contents of AS protein in the large and middle-dose groups were up-regulated (P <0.01). Conclusion SGFZ flavoring can effectively improve the degree of joint swelling in CIA rats, and its mechanism may be related to the regulation of ES and AS protein expression.