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目的 :采用将集落法和 MTT法结合发展而成的双琼脂 MTT法以克服 MTT法中存在正常细胞干扰测定的缺点。方法 :对人肿瘤细胞及正常细胞株在双琼脂 MTT法和 MTT法光密度、抗癌药抑制率进行比较及临床实体瘤用两种方法的抗癌药抑制率进行比较。结果 :正常细胞在双琼脂 MTT法中生长不良 ,用该两种方法测定临床 2 0例食道癌对抗癌药抑制率 ,比较显示双琼脂 MTT法的抑制率大于 MTT法。结论 :双琼脂 MTT法提高了药敏结果的准确性 ,优于 MTT法。
Objective: To use the double agar MTT method developed by combining the colony method and MTT method to overcome the shortcomings of the normal cell interference assay in the MTT assay. Methods: The comparison of the optical acuity and the inhibition rate of anticancer drugs between MTT and MTT assays on human tumor cells and normal cell lines and the inhibition rates of anticancer drugs in clinical solid tumors using two methods were compared. RESULTS: Normal cells grew poorly in the double agar MTT method. The inhibitory rate of anticancer drugs in 20 cases of esophageal cancer was determined by the two methods. The comparison showed that the inhibition rate of the double agar MTT method was greater than that of the MTT method. Conclusion: The double agar MTT method improves the accuracy of the drug susceptibility results and is superior to the MTT method.