L7811白血病细胞质膜经不连续蔗糖密度梯度离心法和双向聚丙烯酰胺凝胶电泳分析其蛋白组分,发现有Mr约100kD、pI约4.0组分的缺失和Mr约63kD、PI约4.5的组分出现。 材料与方法 一、实验材料 纯系615小鼠移植性腹水型自血病细胞(即L7811白血病细胞,属T淋巴细胞白血病),由中国医学科学院血液研究所引进。对照组为同系615小鼠(由中国医学科学院动物所引进)的胸腺T细胞。 The protein components of L7811 leukemia cell plasma membrane were analyzed by discontinuous sucrose density gradient centrifugation and two-way polyacrylamide gel electrophoresis. It was found that there was a deletion of about 100 kD of Mr, about 4.0 of pI, and a component of Mr of about 63 kD and PI of about 4.5. appear. Materials and Methods First, experimental materials Pure 615 mice transplanted ascites-type autologous blood cells (ie L7811 leukemia cells, a T-lymphocyte leukemia), introduced by the Institute of Hematology, Chinese Academy of Medical Sciences. The control group was thymic T cells of syngeneic 615 mice (imported by the Chinese Academy of Medical Sciences Animals).