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目的观察褪黑素(MT)治疗前后急性百草枯(PQ)中毒大鼠肺组织中核因子-κB(NF-κB)及诱导型一氧化氮合酶(iNOS)表达的变化,探讨它们在百草枯所致肺损伤中的作用。方法将45只SD大鼠随机分为染毒组、MT治疗组和对照组。3d后分别测定三组大鼠血清中一氧化氮(NO)及丙二醛(MDA)含量,同时取大鼠肺组织,采用电泳迁移率改变分析法(EMSA)检测NF-κB活性;RT-PCR检测iNOSm-RNA的表达。结果大鼠肺组织NF-κB活性及iNOSmRNA的表达在染毒组明显高于对照组(P<0.01)。经MT治疗后显著降低(P<0.01),但仍高于对照组(P<0.01)。染毒组大鼠血清中NO及MDA的浓度较对照组明显升高(P<0.01),经MT治疗显著降低(P<0.01)。结论NF-κB及iNOS在百草枯所致大鼠肺损伤中起重要作用;MT能减少染毒大鼠肺组织NF-κB的激活,降低其调控的iNOS活性,减轻染毒大鼠肺组织损伤。
Objective To observe the changes of nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS) expression in lungs of acute paraquat (PQ) poisoned rats before and after treatment with melatonin (MT) The role of lung injury caused. Methods Forty five Sprague-Dawley rats were randomly divided into four groups: untreated group, MT treated group and control group. After 3 days, the content of nitric oxide (NO) and malondialdehyde (MDA) in serum of the three groups were respectively measured. At the same time, the lung tissues of rats were taken out and the activity of NF-κB was detected by electrophoretic mobility shift assay (EMSA) PCR detection of iNOSm-RNA expression. Results The NF-κB activity and iNOS mRNA expression in lung tissue of rats were significantly higher than those of the control group (P <0.01). After MT treatment was significantly lower (P <0.01), but still higher than the control group (P <0.01). The concentrations of NO and MDA in the serum of the treated group were significantly higher than those of the control group (P <0.01), and were significantly decreased by the MT treatment (P <0.01). Conclusion NF-κB and iNOS play an important role in paraquat-induced lung injury in rats. MT can reduce the activation of NF-κB in lung tissue, decrease the iNOS activity and attenuate lung injury .