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目的 通过胚鼠皮质神经元的体外培养 ,观察遭受缺氧刺激时 ,脑源性神经营养因子 (BDNF)对神经元的保护作用。方法 用台盼蓝染色法动态观察培养神经元的存活率及加入BDNF对生理培养状态的皮质神经元的作用 ;同时用四氮唑盐 (MTT)比色法观察不同缺氧时间实验组及对照组神经元活性的差异。结果 BDNF对正常皮质神经元有维持存活效应 ;对缺氧神经元有确切保护作用 ,该保护作用有时间依赖性 ,缺氧前2 4h加入BDNF对缺氧神经元的保护效果较缺氧即刻加入为好。结论 体外实验证实BDNF可维持皮质神经元存活 ,并可减轻皮质神经元所遭受的缺氧损害
Objective To observe the protective effects of brain derived neurotrophic factor (BDNF) on neurons in vitro under hypoxia stimulation by culturing embryonic rat cortical neurons in vitro. Methods The survival rate of cultured neurons and the effect of adding BDNF on cultured cortical neurons were observed by trypan blue staining. At the same time, MTT assay was used to observe the effects of different time of hypoxia on experimental group and control group Group differences in neuronal activity. Results BDNF had the survival and survival effects on normal cortical neurons and had definite protective effect on hypoxic neurons. The protective effect of BDNF was time-dependent. The protective effects of BDNF on hypoxic neurons at 24 h before hypoxia were added immediately As well Conclusion In vitro experiments confirmed that BDNF can maintain the survival of cortical neurons and reduce the hypoxic damage suffered by cortical neurons