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背景:重组人促红细胞生成素是一种糖蛋白,近年的研究表明其对神经细胞的许多功能活动均具有调节作用。目的:观察不同浓度重组人促红细胞生成素对神经干细胞体外培养增殖的影响。方法:提取新生SD大鼠神经干细胞,用含不同浓度(5,50,500U/mL)重组人促红细胞生成素和20μg/L碱性成纤维细胞生长因子的无血清培养基进行培养,以不含重组人促红细胞生成素无血清培养基为对照组。细胞培养7d后计算神经干细胞克隆形成率,培养10d后计数NSE和GFAP免疫阳性细胞数。结果与结论:添加重组人促红细胞生成素组细胞增殖较快,最终神经球的数量多于对照组,以50U/mL重组人促红细胞生成素组作用显著;50U/mL重组人促红细胞生成素组的生长速度显著快于对照组。50U/mL重组人促红细胞生成素组中NSE和GFAP免疫阳性细胞明显多于对照组(P<0.01)。结果表明重组人促红细胞生成素对神经干细胞体外培养增殖有促进作用,尤以适中浓度(50U/mL)作用更加明显。
BACKGROUND: Recombinant human erythropoietin is a glycoprotein and recent studies have shown that it regulates many of the functional activities of nerve cells. Objective: To observe the effect of different concentrations of recombinant human erythropoietin on proliferation and proliferation of neural stem cells in vitro. Methods: Neural stem cells of neonatal SD rats were isolated and cultured in serum-free medium containing recombinant human erythropoietin and 20μg / L basic fibroblast growth factor at different concentrations (5, 50, 500U / mL) Recombinant human erythropoietin serum-free medium as a control group. The colony formation rate of neural stem cells was calculated 7 days after cell culture, and the numbers of NSE and GFAP immunoreactive cells were counted after 10 days of culture. RESULTS AND CONCLUSION: The cells proliferated rapidly with the addition of recombinant human erythropoietin and the number of the final neurospheres was more than that of the control group. The effect of 50 U / mL recombinant human erythropoietin was significant. The 50 U / mL recombinant human erythropoietin Group growth rate was significantly faster than the control group. The number of NSE and GFAP immunoreactive cells in 50U / mL recombinant human erythropoietin group was significantly more than that in control group (P <0.01). The results showed that recombinant human erythropoietin can promote the proliferation of neural stem cells in vitro, especially at a moderate concentration (50U / mL).