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目的建立苣荬菜地上部位总黄酮含量的测定方法,比较不同产地和同一产地不同采收时间对苣荬菜地上部位总黄酮含量的影响。方法对6个不同来源和5个不同采时间的苣荬菜地上部位超声提取,分别用紫外可见分光光度法测定提取物中总黄酮含量。结果由于产地、采收时间的不同,总黄酮的含量差异较大,其中天津宝坻中总黄酮含量最高,宁夏银川在各个产地中的黄酮含量最低,在甘肃武威样品中批号为20130801的总黄酮含量最低。结论本法可以作为苣荬菜地上部位总黄酮的含量测定方法,产地和采收时间对苣荬菜地上部位总黄酮含量影响较大,实验结果可用于指导筛选苣荬菜优良产地和合理采集时间。
Objective To establish a method for the determination of total flavonoids in the aerial part of Brasenia schreberi and compare the effects of different harvesting time and harvest time on the total flavonoids content in the aboveground part of Brasenia schreberi. Methods Ultrasound extraction of the aerial parts of Cichlorette chinense from 6 different sources and 5 different sampling times was carried out. The content of total flavonoids in extracts was determined by UV-Vis spectrophotometry. Results The content of total flavonoids differed greatly due to different producing areas and harvesting times. Among them, the content of total flavonoids in Tianjin Baodi was the highest, while the content of flavonoids in Yinchuan of Ningxia was the lowest. The total flavonoid content of 20130801 lowest. Conclusion This method can be used as a method for determination of the content of total flavonoids in the aerial parts of B. japonica. The origin and the harvesting time have a great influence on the content of total flavonoids in the aerial part of the B. subtropatae. The experimental results can be used to guide the selection of the excellent origin and the reasonable collection time .