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通过对N~+离子注入棉子后M_1代植株第二真叶过氧化物酶同功酶酶谱的分析,探讨了酶谱变异与离子注入量及农艺性状损伤的关系。结果表明:N~+离子注入引起酶带数目和活性的明显变化,共出现12种类型的谱带,最多可见5条,最少为1条,并且在阴极显示一条3A弱带,此带在4×10~(16)N~+/cm~2注入量中出现的频率最高。1A和2A带消失的频率与离子注入量呈正相关。具有3A带植株的苗高,第一真叶宽,第一真叶叶面积,铃数和茎粗均显著的高于无3A带的植株。酶谱变化频率的高低在一定程度上反映了离子注入量和性状损伤程度的大小。
Through the analysis of peroxidase isoenzymes of the second true leaf of M 1 plants after N ~ + ion implantation, the relationship between the variation of zymogram and ion implantation amount and agronomic trait was discussed. The results showed that: N ~ + ion implantation caused a significant change in the number and activity of the enzyme band, a total of 12 types of bands, a maximum of 5, with a minimum of 1, and in the cathode shows a weak 3A band in 4 × 10 ~ (16) N ~ + / cm ~ 2 injection frequency appears the highest. The frequency of disappearance of 1A and 2A bands was positively correlated with ion implantation. The seedling height, the first true leaf width, the first true leaf area, the bolls number and the stem diameter of the plants with 3A belt were significantly higher than those without the 3A belt. To some extent, the frequency of zymogram changes reflect the amount of ion implantation and the extent of damage traits.