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应用经克隆了的已知功能或有潜在功能的 DNA序列 ,即候选基因 ,作为分子标记 ,在中 15 6 /谷梅 2号 F8重组自交系群体中进行水稻抗稻瘟病 QTL的分析。大部分候选基因在水稻染色体上成簇分布 ,并且位于已知抗病基因簇区域。应用复合区间法检测到 1个调控病斑大小和 1个调控病斑数量的 QTL,前者位于第 1染色体 CG36 a～ RM2 12区间 ,贡献率为4.17% ,抗性等位基因来自父本谷梅 2号 ;后者定位于第 2染色体 CG18a～RM2 6 3区间 ,贡献率为 6 .2 5 % ,抗性等位基因来自母本中 15 6。同时检测到 2对控制病叶面积和 1对控制病斑大小的基因互作。这些 QTL和互作基因涉及抗性基因同源序列、离子通道调控子以及编码致病相关蛋白和几丁质酶的基因 ,表明候选基因的应用有助于揭示 QTL的功能。玉米锈病抗性基因 Rp1与稻瘟病抗性有关 ,提示了利用水稻这个模式作物来克隆较大基因组中有利基因的可能性
The cloned QTLs for resistance to rice blast disease were analyzed using a cloned known or potential DNA sequence, a candidate gene, as a molecular marker in a population of F8 recombinant inbred lines of Zhongjiu 6 / Gumei 2. Most of the candidate genes are clustered in rice chromosomes and located in the known resistance gene cluster region. A QTL for controlling the size of the spot and the number of a regulated lesion was detected by the composite interval method. The former was located on the CG36 a ~ RM2 12 interval of chromosome 1, with a contribution rate of 4.17%. The alleles of resistance were from the parents Gu Mi 2 The latter locates on the second chromosome CG18a ~ RM2 6 3 interval, the contribution rate was 6.52%, and the resistance allele was from the female parent 15 6. At the same time, two pairs of genes controlling the diseased leaf area and one pair controlling the size of the lesion were detected. These QTLs and their interaction genes involved in the resistance gene homologues, ion channel regulators and genes encoding pathogenicity-related proteins and chitinase, indicating that the application of candidate genes can help reveal the function of QTLs. The Rp1 gene is associated with rice blast resistance, suggesting the possibility of using cloned rice as a model crop to clone favorable genes in larger genomes