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近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75～1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2±29.3毫克/100毫升。四、讨论了不同类型高脂蛋白血症中脂蛋白的异常及分型在临床应用上的意义。
In recent years, the incidence and mortality of coronary heart disease is high, and the incidence of middle-aged and young people is also increasing. Therefore, coronary heart disease has become a major issue in modern medical research. The author initially established a repeatable, simple, cost-effective, suitable for clinical laboratory lipoprotein electrophoretic staining of pre-dyeing method in order to provide more clues to the classification of hyperlipoproteinemia. First, with Sudan Black B petroleum ether - saturated ethanol-saturated solution of serum first, after containing 1% albumin in TEB (trimethylolum chloride - ethylenediaminetetraacetic acid - boric acid) buffer, the voltage 160V, current 0.75 ~ 1.O mA / filter paper, electrophoresis 16 hours, make 40 microliters of normal serum appear on the filter paper in addition to the origin there are three pigmented zone, that is, β-lipoprotein, pre-β-lipid Protein and alpha-lipoprotein. Normal fat meal, in the origin and β-lipoprotein chylomicrons and pre-β-lipoprotein “tail” zone appears. Second, dozens of patient outpatient serum, electrophoresis were observed 20 times the repeatability. Results: The β, β and α-lipoproteins were accordingly 43. O ± O.42 (S.E.)%, 33.7 ± 0.52 (S.E.)% and 23.4 ± 0.47 (S.E.)%. Third, the determination of 33 (18 males and 15 females), mean age 43.7 years, the general health of non-lipid metabolic diseases of the faculty of the lipoprotein: α-, β- and pre-β- The corresponding lipoproteins were 187 ± 42 mg / 100 ml, 241.3 ± 34.2 mg / 100 ml and 75.2 ± 29.3 mg / 100 ml. Fourth, discuss the different types of hyperlipoproteinemia in lipoprotein abnormalities and classification of clinical significance.