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以葡萄砧木‘1103P’为试材,筛选获得了4个受盐强烈诱导的R2R3-MYB基因,系统进化分析表明其编码蛋白属于不同的拟南芥R2R3-MYB亚组。该4个基因在根、茎、叶和果实中呈组成型表达,但具有不同的表达水平,尤其是VvMYB112在葡萄根系中具有较高的表达水平。在4个基因中,VvMYB112能响应100～200 mmol·L-1高盐处理。此外,VvMYB112和VvMYB15也能被聚乙二醇(PEG6000)和低温诱导,而VvMYB107和VvMYB87对PEG6000和低温不敏感或受其抑制。表明这4个基因可能通过不同的途径介导抗盐性。
Four grape rootstock R2R3-MYB genes induced by salt were screened using the grape rootstock ’1103P’ as a material. Phylogenetic analysis indicated that the encoded proteins belong to different Arabidopsis R2R3-MYB subgroups. The four genes were constitutively expressed in roots, stems, leaves and fruits, but with different expression levels. In particular, VvMYB112 had a higher expression level in grape roots. Among the four genes, VvMYB112 responded to 100 ~ 200 mmol·L-1 high salt treatment. In addition, VvMYB112 and VvMYB15 were also induced by polyethylene glycol (PEG6000) and hypothermia, while VvMYB107 and VvMYB87 were insensitive to or inhibited by PEG6000 and hypothermia. The results indicated that these four genes may mediate salt resistance through different pathways.