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对不同基因组的野生稻愈伤组织进行了超低温保存的研究，主要结果如下：①野生稻愈伤组织经过预培养→预处理→冰冻降温→液氮保存→快速解冻的超低温保存，冻后细胞存活率最高可达８７．９％。②１０％ＤＭＳＯ＋８％葡萄糖为最佳冰冻保护剂。降温程序为０℃→－１０℃，１５ｍｉｎ→－４０℃，６０ｍｉｎ→液氮（ＬＮ）。③普通野生稻、宽叶野生稻、疣粒野生稻获得了冻后再生植株。④疣粒野生稻解冻后形成旺盛胚性愈伤组织，并通过体细胞胚胎发生途径再生出大量植株。
The cryopreservation of wild calli in different genomes was carried out. The main results are as follows: ① Wild calli were pre-cultured, pretreated, frozen, cooled, stored in liquid nitrogen and rapidly thawed for cryopreservation, Rate up to 87.9%. ② 10% DMSO + 8% glucose as the best cryoprotectant. Cooling program is 0 ℃ → -10 ℃, 15min → -40 ℃, 60min → liquid nitrogen (LN). ③ common wild rice, broad-leaved wild rice, wart wild rice obtained after the frozen plant regeneration. ④ Warty wild rice formed a strong embryogenic callus after thawing, and regenerated a large number of plants through somatic embryogenesis.