对人工选育的第 3代中国对虾和选育的第 1代凡纳对虾进行个体人工感染WSSV后 ,选取感染WSSV十余天但仍健康的对虾为实验材料 ,用 2 2 0个随机引物进行RAPD分析 ,得到抗病性状相关的特异性遗传标记 99个。其中中国对虾抗病组特异性片段出现了 18个 ,片段大小在 46 0～ 2 30 5bp之间 ;凡纳对虾抗病组特异性片段产生 81个 ,片段大小在 435～ 2 2 87bp。 77个引物在两种对虾的抗病组扩增出特异性遗传标记 ,其中有 4个引物各获得了三个特异性片段 ,有 13个引物各获得了两个特异性片段 ,其余 6 1个引物各获得了 1个特异性片段。 After the artificially selected third-generation Chinese shrimp and the first generation P. vannamei were artificially infected with WSSV, 20 healthy shrimp infected with WSSV were selected as the experimental materials and randomly selected with 220 random primers RAPD analysis showed that there were 99 specific genetic markers related to disease resistance. Among them, there were 18 specific fragments in P. chinensis disease resistance group with the fragment sizes of 46 0-2 30 5 bp. There were 81 specific fragments of P. vannamei disease resistance group ranging in size from 435 bp to 2 87 bp. 77 primers amplified specific genetic markers in the resistance group of two species of shrimp, among which 4 primers obtained three specific fragments, 13 primers each obtained two specific fragments, and the remaining 61 Primers each obtained a specific fragment.