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目的探讨丙泊酚及神经节苷脂(GM1)对SD幼鼠认知功能及脑神经细胞caspase-3表达的影响。方法 17～18日龄SD大鼠77只,体重33～42g,随机分为7组(每组11只),分别腹腔注射生理盐水10ml/kg(NS组)、丙泊酚50mg/kg(P50组)、丙泊酚100mg/kg(P100组)、丙泊酚200mg/kg(P200组)、神经节苷脂10mg/kg(G组)、丙泊酚100mg/kg+神经节苷脂10mg/kg(P100G组)、丙泊酚200mg/kg+神经节苷脂10mg/kg(P200G组),一次性或分次腹腔注射当天药量,连续6d。前5d每天用Morris水迷宫测试其空间学习能力,第6天撤去平台测试其记忆能力。测试结束后处死大鼠,断头取脑,每组随机选取8只应用免疫组化法检测caspase-3的表达,余下3只取新鲜脑组织进行Westernblotting检测caspase-3蛋白表达。结果在水迷宫实验中,与NS组比较,P100组、P200组和P200G组寻找平台潜伏期显著延长,穿越平台次数明显减少(P<0.05);与P100G组比较,P100组寻找平台潜伏期显著延长,穿越平台次数明显减少(P<0.05)。免疫组化结果显示,P100组、P200组、P100G组及P200G组caspase-3表达明显高于NS组(P<0.05)。Westernblotting结果显示,P100G组caspase-3蛋白表达明显低于P100组(P<0.05),而P200G组和P200组间差异无统计学意义。结论较大剂量的丙泊酚可引起caspase-3表达明显增高,影响幼鼠的认知功能;GM1能明显抑制大剂量丙泊酚引起的caspase-3的表达增加,抑制神经细胞凋亡,减轻幼鼠的认知功能障碍。
Objective To investigate the effects of propofol and ganglioside (GM1) on the cognitive function and the expression of caspase-3 in SD rats. Methods Totally 77 SD rats aged 17-18 days weighing 33-42g were randomly divided into 7 groups (n = 11). The rats in each group were injected intraperitoneally with normal saline (10ml / kg NS group) and propofol 50mg / kg (P100 group), propofol 200 mg / kg (P200 group), ganglioside 10 mg / kg (Group G), propofol 100 mg / kg + ganglioside 10 mg / kg (P100G group), propofol 200mg / kg + ganglioside 10mg / kg (P200G group), once or subcutaneously intraperitoneal injection dose for 6 days. The first 5 days with Morris water maze to test their spatial learning ability, the first 6 days to remove the platform to test its memory. After the test, the rats were sacrificed, and the brain was decapitated. The expression of caspase-3 in each group was detected by immunohistochemistry. The remaining 3 brains were taken for Western blotting to detect the expression of caspase-3. Results In the water maze test, compared with the NS group, P100, P200 and P200G groups significantly prolonged the platform latency and decreased the number of crossing the platform (P <0.05). Compared with the P100G group, the latency of the P100 group looking for the platform was significantly prolonged, The number of crossing the platform was significantly reduced (P <0.05). The results of immunohistochemistry showed that the expression of caspase-3 in P100, P200, P100G and P200G groups was significantly higher than that in NS group (P <0.05). Western blotting showed that the expression of caspase-3 in P100G group was significantly lower than that in P100 group (P <0.05), but there was no significant difference between P200G group and P200 group. Conclusions Higher doses of propofol can significantly increase the expression of caspase-3 and affect the cognitive function of young rats. GM1 can significantly inhibit the increase of caspase-3 expression induced by high-dose propofol and inhibit the apoptosis of neurons Cognitive dysfunction in young rats.