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目的 为了解 p16、p15基因与中国人脑肿瘤遗传易感性的关系。 方法 应用PCR、银染PCR SSCP及免疫组化法对 88例脑肿瘤及相应手术切除正常组织DNA标本 p16、p15基因进行研究。 结果 结果显示在不同病理分级脑胶质瘤中p16、p15基因缺失率分别为Ⅰ -Ⅱ级 4%、0 % ,Ⅲ级 3 2 %、2 8% ,Ⅳ级 3 6 %、2 7% ,p16、p15基因缺失主要见于Ⅲ -Ⅳ级肿瘤中 ;脑转移瘤中也检出缺失该基因 ( 2 /3 ) ,而原发性非胶质细胞瘤 (脑膜瘤、垂体瘤、听神经瘤等 )中未见p16、p15基因缺失 ,各类肿瘤标本PCR SSCP分析均未见 p16、p15基因点突变。 结论 表明国人脑瘤中p16、p15基因异常以缺失为主 ,p16、p15基因缺失在脑胶质瘤中发生频率较高 ,促进肿瘤细胞由良性向恶性演进 ,而对良性非胶质细胞瘤的发病没有作用。
Objective To understand the relationship between p16 and p15 gene genetic susceptibility in Chinese human brain tumors. Methods The DNA samples p16 and p15 of 88 cases of brain tumors and their corresponding normal tissues were studied by PCR, silver staining and PCR SSCP and immunohistochemistry. The results showed that the deletion rates of p16 and p15 genes in gliomas with different histological grade were 4%, 0%, 32%, 28%, 36%, 27% The deletion of p16 gene and p15 gene were mainly found in grade Ⅲ-Ⅳ tumors. The gene deletion was also found in brain metastases (2/3). However, the primary non-gliocytoma (meningioma, pituitary tumor, acoustic neuroma, etc.) No p16, p15 gene deletion, all kinds of tumor specimens PCR SSCP analysis showed no p16, p15 gene point mutation. The conclusion shows that the abnormalities of p16 and p15 genes in human brain tumor are mainly absent, while the deletion of p16 and p15 genes is more frequent in glioma and promotes the progression of benign to malignant tumor cells. However, for the patients with benign non-gliocytoma The incidence has no effect.