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螺旋藻作为保健食品或食品添加剂被广泛的人工培养并被制成片状和粉状广泛应用。生物反应器是一个进行涉及到生物或生物活性物质生产的容器。用光生物反应器高密度培养微藻也成为国内外研发的热点。中国养殖的螺旋藻主要为钝顶螺旋藻(Spirulina platensis)。本研究中,以钝顶螺旋藻为试材,用三角瓶和平板式光生物反应器培养,并设计不同的培养条件(添加不同浓度的葡萄糖和抗氧化剂,设置不同通气模式和光照条件),探讨其培养工艺参数的优化。结果显示,在三角瓶中,分别适当添加葡萄糖、Na2S2O3和Na2SO3,均可显著提高钝顶螺旋藻的生物量,其最适浓度分别为3、3和2 g/L;光反应器培养下,随着通气处理强度的上升,藻生物量显著增加;而逐步增大通气强度的处理下,藻生物量促进效果则更为明显;单面光源(4 500 lx)培养下的藻生物量要显著高于双面光源(9 000 lx)培养的,但是先单面光源培养,在微藻进入对数期生长时,转变为双面光源培养,其培养产量更高。进一步研究表明,与Na2S2O3相比,Na2SO3与葡萄糖组合效果更好,进一步对各条件优化显示,在葡萄糖浓度4 g/L和Na2SO3浓度3 g/L时,藻生物量达到最大值。上述研究表明,添加葡萄糖、Na2SO3、逐步增加通气强度的模式、先单面光源后双面光源培养的光照模式可明显促进钝顶螺旋藻的产量,综合优化的培养条件下,该藻体的平均生长速率和生物量可高达0.85 g DW/(L·d)和10.02 g DW/L。本生物反应器还具有动力消耗小、占地面积小、造价相对便宜、清洗方便、结构简单和容易在室内扩大规模培养等优点。本研究为扩大螺旋藻的培养规模提供了理论依据和技术方法。
Spirulina is widely used as a health food or food additive by being extensively cultivated and made into flakes and powder. Bioreactors are vessels that carry out the production of biological or bioactive substances. High density cultivation of microalgae with photobioreactors has also become a hot spot at home and abroad. Spirulina cultured in China is mainly Spirulina platensis. In this study, Spirulina platensis was used as a test material, flask and plate photobioreactor culture, and design different culture conditions (different concentrations of glucose and antioxidants, set different ventilation modes and lighting conditions), to explore Its culture process parameters optimization. The results showed that glucose, Na2S2O3 and Na2SO3 were added into the flask to increase the biomass of Spirulina platensis, the optimum concentrations were 3, 3 and 2 g / L, respectively. Under the photoreactor culture, The biomass of algal biomass increased significantly with the increase of ventilation intensity, while the biomass of algal biomass increased with the increase of aeration intensity. The biomass of algae under single-sided light source (4 500 lx) Higher than the double-sided light source (9 000 lx) cultured, but the first single-sided light source culture, microalgae into the logarithmic growth, the conversion to double-sided light source culture, the higher the cultivation yield. Further studies showed that the combination of Na2SO3 with glucose was better than that of Na2S2O3, and further optimized for each condition showed that the biomass of algae reached its maximum when glucose concentration was 4 g / L and Na2SO3 concentration was 3 g / L. The above studies show that adding glucose, Na2SO3 and gradually increasing the aeration intensity mode, the light pattern of the double-sided light source culture after the single-sided light source can obviously promote the production of Spirulina platensis. Under the optimized culture conditions, the average Growth rates and biomass can be as high as 0.85 g DW / (L · d) and 10.02 g DW / L. The bioreactor also has the advantages of small power consumption, small area, relatively cheap cost, convenient cleaning, simple structure and easy scale expansion in a room. This study provides a theoretical basis and technical methods for expanding the scale of Spirulina cultivation.