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目的:观察豚鼠膀胱cajal样细胞形态学特点并结合该细胞具有的特殊功能,探讨cajal样细胞在膀胱组织中的类型。方法:豚鼠膀胱经手术取材后制成冷冻切片和体外培养膀胱cajal样细胞,采用间接免疫荧光染色后在激光共聚焦显微镜观察cajal样细胞形态学特点,然后将体外培养的膀胱cajal样细胞应用F1uo-4AM标记细胞内钙离子浓度含量,在激光共聚焦显微镜下观察cajal样细胞钙离子振荡功能。结果:膀胱组织冷冻切片和体外cajal样细胞培养均可见单体cajal样细胞和二聚体cajal样细胞,体外培养的两种类型的cajal样细胞具有钙离子振荡功能。结论:豚鼠膀胱组织中存在单体和二聚体两种不同类型的cajal样细胞。
OBJECTIVE: To observe the morphological characteristics of cajal-like cells in the bladder of guinea pigs and to explore the types of cajal-like cells in the bladder tissue combined with the special functions of the cells. Methods: The bladder of Guinea pigs were made into frozen sections and cultured in vitro. The Cajal-like cells were cultured in vitro. The morphological characteristics of Cajal-like cells were observed by laser scanning confocal microscopy after indirect immunofluorescence staining. Then the Cajal- -4AM labeled intracellular calcium concentration, under confocal laser scanning microscope Cajal-like cells calcium oscillation. RESULTS: Both cytoplasmic cajal-like cells and dimeric cajal-like cells were observed in both frozen section and cajal-like cell culture in vitro. Two types of cajal-like cells cultured in vitro had calcium oscillation. CONCLUSION: Two different types of cajal-like cells, monomeric and dimeric, exist in the bladder tissue of guinea pigs.