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本文研究了体外培养恶性疟原虫上清液刺激淋巴细胞产生免疫球蛋白的结合特异性。并对疟疾的多克隆淋巴细胞活化作用在产生自家抗体的可能作用进行了讨论。采用10名无疟史的健康成年高加索供血员作血样,加入淋巴细胞分离剂(Nygaard),经离心子交界处收集单核细胞,以1×10~6/ml为最终浓度,置于多井平板培养,每个供体细胞各进行二系列的三重培养,一系列加入含有小牛血清和抗菌索的恶性疟原虫培养上清液,另一系列加入对照培养上清液,
In this paper, we studied the binding specificity of Plasmodium falciparum supernatant stimulated lymphocytes to produce immunoglobulin in vitro. The possible role of polyclonal lymphocyte activation in malaria in generating autoantibodies is also discussed. Ten healthy males with no history of malaria were used as blood samples. Nygaard was added and mononuclear cells were collected through the centromere junction. The cells were placed in multi-wells at a concentration of 1 × 10 ~ 6 / ml Each of the donor cells was subjected to two series of triple culture, a series of culture supernatants of Plasmodium falciparum containing bovine serum and antibacterial cord were added, another series of control culture supernatants were added,