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本实验利用针对非肌性αactinin抗体检测阿霉素肾病大鼠肾组织内αactinin的分布及蛋白水平,采用逆转录 聚合酶链反应(RT PCR)法检测阿霉素肾病大鼠肾组织内αac tinin 4mRNA水平的表达,探讨αactinin在阿霉素肾病大鼠蛋白尿产生中的作用。1 对象和方法1.1 动物和药品 健康?
In this study, the non-muscle α-actinin antibody was used to detect the distribution and protein level of α-actinin in adriamycin-induced nephropathy rats. The expression of α-acinin in renal tissue of adriamycin-induced nephropathy rats was detected by reverse transcriptase-polymerase chain reaction (RT PCR) 4 mRNA expression levels, explore the role of αactinin in adriamycin nephropathy rats proteinuria. 1 objects and methods 1.1 animals and medicines health?