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采用RT-PCR技术,克隆了小麦胞质顺乌头酸酶基因(cACO)部分cDNA序列.该cDNA序列长1368bp,编码456个氨基酸,GenBank登录号为GU475062.半定量RT-PCR结果表明,在生理型不育和可育花药发育的单核早期至三核期,cACO基因的表达水平均表现为先升后降;在生理型不育花药发育的单核晚期cACO基因表达水平与同期可育花药相比显著升高,到二核期和三核期明显降低,ACO酶活性变化表现出相同趋势.这反映出在小麦生理型不育系中,cACO基因在花药败育关键期异常表达可能影响了花药发育过程中正常的能量供应和物质代谢,导致花粉发育能量不足和所需物质匮乏,从而导致了非遗传型花药败育现象.
The cDNA sequence of cACO gene was cloned by RT-PCR and the cDNA sequence was 1368bp in length and encoded 456 amino acids with GenBank accession number GU475062. Semi-quantitative RT-PCR results showed that the The expression levels of cACO gene in early mononuclear to trigenetic stages of physiological sterile and fertile anther all increased first and then decreased. The cACO gene expression level in the late stage of mononuclear nucleus with physiological sterile anthers was similar to that in the same period Anther significantly increased compared with that of the anther, and decreased to the second and third nuclei significantly, and ACO activity showed the same trend, which indicated that the cACO gene may be abnormally expressed in the key stage of anther abortion in wheat physiological CMS lines Affecting normal energy supply and material metabolism during anther development, resulting in insufficient developmental energy of pollen and lack of required material, resulting in the abortion of non-genetic anther.