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用高效液相色谱仪,以甲硝唑为内标,建立了一种简便、快速、灵敏的内标法,测定了8名年青健康志愿者的血药浓度。本法的平均回收率为100.32%,RSD为3.02%,最低检出限为0.2μg/ml。
A simple, rapid and sensitive internal standard method was established by high performance liquid chromatography and metronidazole as an internal standard. The blood concentration of eight young healthy volunteers was determined. The average recovery of this method was 100.32%, RSD 3.02%, the minimum detection limit of 0.2μg / ml.