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应用 7， 12-二甲基苯蒽 /巴豆油二阶段诱癌法诱发小鼠皮肤乳头状瘤，观察口服芳维 A酸乙酯 14天的消退作用，利用原位 DNA末端标记技术 (TUNEL法 )和电镜检查结合计算机图像分析对表皮细胞凋亡进行检测。结果，在第 14天，对照组和各服药组小鼠皮肤乳头状瘤平均直径增殖率分别为 125.3％、 66.7％、 79.2％、 91.6％；细胞凋亡指数分别为： 2.3％、 37.7％、 32.3％、 20.5％；服药组与对照组有显著性差异 (P< 0.001)，其消退作用与剂量呈依赖关系，并与细胞凋亡程度正相关 (r=0.89)。结果表明，芳维 A酸乙酯对 DMBA/巴豆油诱发的小鼠皮肤乳头状瘤有明显的消退作用，其作用与诱导异常增殖表皮细胞凋亡有关。
Application of 7,12-dimethylbenz (a) anthracene / croton oil two-stage induced by inducing skin papilloma in mice, observation of oral administration of arvoviride 14 days of regression, the use of in situ DNA end labeling (TUNEL method ) And electron microscopy combined with computer image analysis of epidermal cell apoptosis. The results showed that on day 14, the average proliferative rate of papilloma of mice in the control group and each medication group were 125.3%, 66.7%, 79.2% and 91.6%, respectively; the apoptotic index was 2.3% and 37.7% 32.3% and 20.5% respectively. There was a significant difference between the medication group and the control group (P <0.001). The effect of dose reduction was dose-dependent and positively correlated with the degree of apoptosis (r = 0.89). The results showed that Fang Fang A ethyl acetate DMBA / croton oil-induced mouse dermal papilloma obvious regressive role, and its role in the abnormal proliferation of epidermal cells induced apoptosis.