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目的:研究中药厚朴提取物和厚朴酚与厚朴酚对白血病细胞的增殖抑制和诱导凋亡作用。方法:用MTT、Annex-inV、凋亡DNA Ladder等实验,观察和厚朴酚与厚朴酚对白血病K562、HL-60和U937细胞抑制增殖和诱导凋亡的作用。结果:和厚朴酚实验组对K562、HL-60和U937有增殖抑制作用,呈剂量-时间依赖性,且对K562、HL-60和U937有诱导凋亡作用;厚朴酚对HL-60有增殖抑制作用,并呈剂量-时间依赖性,但与和厚朴酚联合用药无协同作用。结论:和厚朴酚与厚朴酚对白血病细胞株均有增殖抑制作用,且呈剂量-时间依赖性,但二者之间无联合协同作用。
OBJECTIVE: To study the effects of magnolol extract and magnolol on proliferation and apoptosis of leukemia cells. Methods: The effects of honokiol and magnolol on the proliferation and apoptosis of leukemia K562, HL-60 and U937 cells were observed by MTT assay, Annex-inV assay and DNA Ladder assay. Results: The experimental group of honokiol could inhibit the proliferation of K562, HL-60 and U937 cells in a time-and dose-dependent manner and induce the apoptosis of K562, HL-60 and U937 cells; A proliferation inhibition, and a dose-time-dependent, but with honokiol combined with no synergistic effect. CONCLUSION: Both honokiol and magnolol can inhibit the proliferation of leukemia cell lines in a dose-and time-dependent manner, but there is no synergistic effect between them.