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研究利用高密度富硒螺旋藻(Se-SP)细胞通过生物转化制备纳米元素硒(Nano-Se)的可行性,观察Nano-Se在体外对氧自由基的清除作用。用梯度离心分选Nano-Se,原子力显微镜(AFM)、透射电镜(TEM)及X-射线能谱(EDX)联用表征纳米粒中的元素硒形态,电感耦合等离子质谱仪(ICP-MS)测定Nano-Se中的硒含量,化学发光方法检测Nano-Se在体外对超氧自由基和羟自由基的清除作用。结果发现,Nano-Se主要由元素硒构成,形态呈球形,73%的纳米粒子直径大小分布在(61±17)nm范围。Nano-Se在体外对两种氧自由基的最大清除率分别为:30.1%和27.6%,相应的EC50分别为:0.8μg/ml和2.2μg/ml。相同剂量时,Nano-Se对氧自由基的清除作用比硒代蛋氨酸(Se-Met)及Se-SP中其它含硒活性成分更强。结果提示,利用高密度Se-SP可诱导Nano-Se的大量生成,Se-SP转化的Nano-Se可能是一种新的抗氧化硒形态,其作用机制和体内生物活性有待深入研究。
To investigate the feasibility of Nano-Se biosynthesis by high-density selenium-rich spirulina (Se-SP) cells and to observe the scavenging effect of Nano-Se on oxygen free radicals in vitro. The elemental selenium forms in the nanoparticles were characterized by gradient centrifugation and Nano-Se, atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray energy dispersive spectroscopy (EDX). ICP- The content of selenium in Nano-Se was determined. The chemiluminescence method was used to detect the scavenging effect of Nano-Se on superoxide and hydroxyl radicals in vitro. The results showed that Nano-Se was mainly composed of elemental selenium and spherical in shape. The diameter of 73% of the nanoparticles was distributed in the range of (61 ± 17) nm. The maximum clearance rate of Nano-Se against the two oxygen free radicals in vitro was 30.1% and 27.6%, respectively, with corresponding EC50s of 0.8 μg / ml and 2.2 μg / ml, respectively. At the same dosage, the scavenging effect of Nano-Se on oxygen free radicals is stronger than other selenium-containing active ingredients in selenomethionine (Se-Met) and Se-SP. The results suggest that high-density Se-SP can induce the massive production of Nano-Se. Se-SP converted Nano-Se may be a new antioxidant selenium form. Its mechanism of action and in vivo biological activity need further study.