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目的 :研究养阴方对培养人脐静脉内皮细胞抗凝和纤溶等功能的影响。方法 :采用血清药理学方法观察养阴方对培养人脐静脉内皮细胞抗凝、纤溶及抗自由基损伤的作用 ,并与丹参作比较。结果 :养阴方具有显著增高 6酮前列腺素 F1α(6 keto PGF1α)含量 ;提高组织型纤溶酶原激活物 (t PA)的活性 ,抑制组织型纤溶酶原激活抑制物 (PAI)活性 ;提高超氧化物歧化酶 (SOD)活力及降低丙二醛 (MDA)含量的作用。养阴方抗凝、纤溶及抗自由基损伤作用显著优于丹参。结论 :养阴生津方药抗栓溶栓的作用机制与活血化瘀类药不完全相同 ,其主要是通过提高细胞抗氧化和抑制脂质过氧化作用来保护细胞免受损伤 ;通过调节抗凝、纤溶作用达到化瘀功效。养阴生津方药治疗血瘀证主要是通过增水行血、濡润脉道、消散瘀凝、滋养脏腑以调节血行等多种作用机制达到消除瘀血的目的。
Objective : To study the effect of Yangyin Decoction on the anticoagulant and fibrinolysis of cultured human umbilical vein endothelial cells. Methods: Serum pharmacological methods were used to observe the effects of Yangyin Decoction on the anticoagulant, fibrinolysis and anti-free radical damage of cultured human umbilical vein endothelial cells, and compared with Salvia miltiorrhiza. Results: Yangyin Decoction significantly increased the content of 6 keto prostaglandin F1α (6 keto PGF1α), increased the activity of tissue plasminogen activator (t PA), and inhibited the activity of tissue plasminogen activator inhibitor (PAI). ; To improve the activity of superoxide dismutase (SOD) and reduce the malondialdehyde (MDA) content. Anti-coagulation, fibrinolysis and anti-free radical injury of Yangyinfang were significantly better than Salvia miltiorrhiza. Conclusion: The mechanism of antithrombotic thrombolysis of Yangyin Shengjin prescription is not completely the same as that of promoting blood circulation and removing phlegm. It is mainly to protect cells from damage by increasing the antioxidation and inhibiting lipid peroxidation of the cells; by regulating anticoagulation, Fibrinolysis achieves its effectiveness. Yangyin Shengjin Recipe for blood stasis syndrome is mainly used to eliminate blood stasis by increasing blood flow, turbulent phlegm, dissolving phlegm, nourishing organs to regulate blood circulation and other mechanisms.