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目的研究机体代谢状况、环境温度和CO2浓度及年龄对昆明小鼠缺氧耐受性的影响。方法选择健康SPF级昆明小鼠进行缺氧耐受性实验。测定不同机体代谢状况[将30只(成年、雌雄各半)昆明小鼠随机分为3组,分别为对照组(生理盐水)、尼可刹米(浓度为0.25 g/ml)处理组和戊巴比妥钠(浓度为3%)处理组,每组10只。采用腹腔注射方式进行一次性染毒]、环境温度[将50只(成年、雌雄各半)昆明小鼠随机分为5组,分别为常温对照(24℃)组和0、20、40、60℃处理组,每组10只]、CO2浓度[将20只(成年、雌雄各半)昆明小鼠随机分为2组,分别为钠石灰处理组和钠石灰未处理组,每组10只)]及年龄(分别选择成年、未成年昆明小鼠各10只,雌雄各半)小鼠的存活时间、总耗氧量和总耗氧率。结果与对照组比较,尼可刹米处理组昆明小鼠的存活时间和总耗氧量均下降,总耗氧率增高;而戊巴比妥钠处理组昆明小鼠的存活时间和总耗氧量均上升,总耗氧率下降,差异均有统计学意义(P<0.01)。昆明小鼠在常温(24℃)与20℃时的存活时间、总耗氧量、总耗氧率间比较,差异无统计学意义;当环境温度较低(0℃)时,小鼠的存活时间和总耗氧量升高,总耗氧率降低,差异均有统计学意义(P<0.01);当环境温度较高(40、60℃)时,小鼠的存活时间和总耗氧量下降,总耗氧率升高,差异均有统计学意义(P<0.01)。随着环境温度的升高,小鼠存活时间和总耗氧量均呈下降趋势,总耗氧率呈上升趋势。与钠石灰未处理组比较,钠石灰处理组昆明小鼠的总耗氧率较高,且由于低张性缺氧,存活时间缩短,总耗氧量降低,差异均有统计学意义(P<0.01)。与未成年昆明小鼠比较,成年昆明小鼠存活时间和总耗氧量下降,总耗氧率升高,差异均有统计学意义(P<0.01)。结论高代谢状态、高温、高CO2浓度、高龄均可降低昆明小鼠对缺氧的耐受性。
Objective To study the effects of metabolic status, ambient temperature, CO2 concentration and age on hypoxia tolerance in Kunming mice. Methods Healthy SPF Kunming mice were selected for hypoxic tolerance test. The metabolic status of different organs was measured. 30 Kunming mice were randomly divided into three groups: control group (normal saline), nicotine (0.25 g / ml) Sodium pentobarbital (3% concentration) treatment group, 10 rats in each group. The mice were randomly divided into 5 groups at normal temperature (24 ℃) and 0,20,40,60 ℃ treatment group, 10 in each group], CO2 concentration [20 (adult, male and female) Kunming mice were randomly divided into two groups, namely sodium lime treatment group and sodium lime untreated group, 10 in each group) ] And age (respectively, adult and juvenile Kunming mice of each 10, male and female mice) survival time, total oxygen consumption and total oxygen consumption rate. Results Compared with the control group, the survival time and the total oxygen consumption of Kunming mice decreased and the total oxygen consumption rate increased in the nicotine-treated Kunming mice. The survival time and the total oxygen consumption of Kunming mice The volume increased, the total oxygen consumption rate decreased, the differences were statistically significant (P <0.01). There was no significant difference in the survival time, total oxygen consumption and total oxygen consumption of Kunming mice at room temperature (24 ℃) and 20 ℃; when the ambient temperature was low (0 ℃), the survival of mice Time and total oxygen consumption increased, the total oxygen consumption rate decreased, the difference was statistically significant (P <0.01); when the ambient temperature is high (40,60 ℃), the survival time of mice and total oxygen consumption Decreased, the total oxygen consumption rate increased, the differences were statistically significant (P <0.01). With the increase of ambient temperature, the survival time and total oxygen consumption of mice showed a downward trend, the total oxygen consumption rate is on the rise. Compared with natrium-lime untreated group, the total oxygen consumption rate of Kunming mice in soda-lime treated group was higher, and due to hypobaric hypoxia, survival time shortened and total oxygen consumption decreased, the difference was statistically significant (P < 0.01). Compared with undeveloped Kunming mice, the survival time and total oxygen consumption of adult Kunming mice decreased, and the total oxygen consumption rate increased, the differences were statistically significant (P <0.01). Conclusion High metabolic state, high temperature, high CO2 concentration and aging can reduce the hypoxic tolerance of Kunming mice.