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目的研究非洛地平(felodipine,Fel)在醛固酮(aldosterone,Ald)诱导的心肌重构中的保护作用及其可能的机制。方法SD大鼠32只随机分为4组(每组n=8),即对照组、Ald组、Ald+Fel组和Ald+螺内酯(spironolac-tone,Spi)组。Ald组:以Ald 18μg/d皮下注射,连续4周;对照组:注射等量生理盐水4周;Ald+Fel组:以Ald18μg/d皮下注射,同时给于Fel 5 mg/(kg.d)灌胃,连续4周;Ald+Spi组:以Ald 18μg/d皮下注射,同时给予Spi20 mg/(kg.d)灌胃,连续4周。比较各组大鼠的左心室质量(LVW)及全心质量(HW)与体质量(BW)的比值,心肌组织匀浆超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,心肌胶原面积(CA),以及心肌细胞凋亡指数(AI)。结果与Ald组相比,对照组、Ald+Fel组和Ald+Spi组大鼠的LVW/BW,HW/BW,CA和AI均减少,对照组和Ald+Spi组大鼠MDA的含量较Ald组减少,SOD的含量较Ald组增加(P<0.01);Ald+Fel组大鼠SOD和MDA的含量与Ald组相比无明显差异。结论Fel能够改善Ald诱导的心肌肥大和心肌细胞凋亡,但不能改善Ald诱导的氧化应激水平的增加。
Objective To study the protective effect of felodipine (Fel) on aldosterone (Ald) induced myocardial remodeling and its possible mechanism. Methods Thirty-two SD rats were randomly divided into 4 groups (n = 8 in each group), namely control group, Ald group, Ald + Fel group and Ald + spironolactone group. Ald group: injected with Ald 18 μg / d subcutaneously for 4 weeks; control group injected with normal saline for 4 weeks; Ald + Fel group injected with Ald 18 μg / The rats in the Ald + Spi group were injected subcutaneously with Ald 18μg / d, while Spi 20 mg / (kg.d) was given orally for 4 consecutive weeks. The ratio of left ventricular mass (LVW), total heart mass (HW) to body weight (BW) and the content of superoxide dismutase (SOD) and malondialdehyde (MDA) Myocardial collagen area (CA), and cardiomyocyte apoptosis index (AI). Results Compared with the Ald group, the LVW / BW, HW / BW, CA and AI in the control, Ald + Fel and Ald + Spi groups decreased, while the MDA content in the control and Ald + Spi groups was lower than that of Ald (P <0.01). The contents of SOD and MDA in Ald + Fel group were not significantly different from those in Ald group. Conclusion Fel can improve Ald-induced cardiac hypertrophy and cardiomyocyte apoptosis, but can not improve the Ald-induced increase in the level of oxidative stress.