论文部分内容阅读
本文采用混合酸酐法和碳二亚胺两种方法制备 rT_3-BSA,并进行了比较,结果混合酸酐法优于碳二亚胺法。采用置换法(rT_3作底物)制备I~(125)-rT_3。本测定法灵敏度5ng/dl,批内变异系数平均5.6±0.23%,批间变异系数11±3.2%,回收率97.8%,抗血清亲和常数为1.6l×109M~(-1)。测定了正常人、甲状腺机能亢进(甲亢)和甲状腺机能减退(甲减)、非甲状腺疾病近1,000例标本的rT_3、T_3、T_4、FT_3、FL_4、TSH 及 T_3/rT_3比值。148名正常成人平均 rT_3为46.9±10.6ng/dl,儿童组rT_3较正常成人组高(P<0.01);甲亢 rT_3(143.3±51.8)明显高于正常(P<0.001);甲减中 rT_3明显低于正常(P<0.01);非甲状腺疾病中 rT_3明显高于正常,T_3、FT_3则明显下降.
In this paper, two kinds of mixed anhydride method and carbodiimide preparation rT_3-BSA, and compared, the results of the mixed anhydride method is better than the carbodiimide method. I ~ (125) -rT_3 was prepared by displacement method (rT_3 as substrate). The sensitivity of this assay was 5 ng / dl, the intra-assay CV was 5.6 ± 0.23%, the inter-assay CV was 11 ± 3.2%, the recovery was 97.8% and the antisera was 1.6l × 109M -1. The ratio of rT_3, T_3, T_4, FT_3, FL_4, TSH and T_3 / rT_3 in normal subjects, patients with hyperthyroidism, hypothyroidism and hypothyroidism was measured. The average rT_3 in 148 normal adults was 46.9 ± 10.6ng / dl, while that in children was higher than that in normal adults (P <0.01). The rT_3 (143.3 ± 51.8) in hyperthyroidism was significantly higher than that in normal controls (P <0.001) Lower than normal (P <0.01). In non-thyroid disease, rT_3 was significantly higher than normal while T_3 and FT_3 decreased significantly.