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目的用荧光原位杂交(fluorescence in situ hybridization,FISH)方法检测肺癌进展组织芯片中Survivin mRNA的表达,并探讨其在肺癌发生、发展中的作用和意义。方法利用FISH方法和组织芯片技术,检测肺原发癌89例,淋巴结转移肺癌12例,肺癌前病变12例和正常肺标本10例中Sur- vivin mRNA的表达情况。结果原发肺癌中有69．7％表达Survivin mRNA,显著高于正常组(P<0．05);癌前病变组Survivin mRNA的表达显著高于正常组 (P<0．05);Survivin mRNA的表达与分化程度、淋巴结转移和临床分期相关(P<0．05)。结论 FISH敏感性强,荧光标记物稳定;组织芯片技术具有高效率、高信息量等优点,在病理学中有很好的应用前景;Survivin mR- NA在肺癌和癌前病变组织中高表达,与进展程度和恶性行为相关,对肺癌的发生、发展起促进作用,为早期诊断、预后估计和治疗提供一定的依据。
Objective To detect the expression of Survivin mRNA in lung cancer tissue samples by fluorescence in situ hybridization (FISH), and to explore the role and significance of Survivin mRNA in lung cancer. Methods FISH and tissue microarray were used to detect the expression of Survivin mRNA in 89 cases of primary lung cancer, 12 cases of lymph node metastatic lung cancer, 12 cases of precancerous lesions and 10 cases of normal lung specimens. Results The expression of Survivin mRNA in primary lung cancer was 69.7%, which was significantly higher than that in normal group (P <0.05). The expression of Survivin mRNA in precancerous lesions was significantly higher than that in normal group (P <0.05) The expression was correlated with the degree of differentiation, lymph node metastasis and clinical stage (P <0.05). Conclusions FISH is sensitive and the fluorescent label is stable. Tissue microarray technology has the advantages of high efficiency, high information content and so on. It has a good prospect in pathology. Survivin mR-NA is highly expressed in lung cancer and precancerous lesions, Progress and malignant behavior related to the occurrence and development of lung cancer play a role in promoting early diagnosis, prognosis and treatment to provide some basis.