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目的:研究甜菜碱对癫痫大鼠海马胶质细胞原纤维酸性蛋白(GFAP)、甘氨酸(Gly)及甘氨酸受体(GlyR)表达的影响。方法:将健康雄性Wistar大鼠随机分为:正常对照组(腹腔注射生理盐水,1.0 mL生理盐水灌胃);癫痫组(腹腔注射戊四氮,1.0 mL生理盐水灌胃);甜菜碱高、中、低浓度组(腹腔注射戊四氮,甜菜碱灌胃);丙戊酸钠组(腹腔注射戊四氮,丙戊酸钠灌胃)。实验结束后大鼠眼眶取血检测血清中同型半胱氨酸的含量;在低温条件下迅速取脑组织,分析Gly含量的变化,免疫荧光检测GFAP的水平,兔疫荧光和Western bloting检测海马区GlyR表达的变化。结果:各组大鼠大发作潜伏期无显著差异(P>0.05),但是甜菜碱治疗组较癫痫组的首次大发作持续时间显著缩短(P<0.01)。癫痫组同型半胱氨酸的含量与正常组比较显著降低(P<0.01),甜菜碱高、低浓度组同型半胱氨酸含量与癫痫组比较明显降低(P<0.05)。癫痫组甘氨酸的含量与正常对照组相比显著下降(P<0.01)。甜菜碱高、中、低浓度组甘氨酸的含量与癫痫组比较显著增高(P<0.01)。免疫荧光检测GFAP结果,癫痫组与正常组比较显著增高(P<0.01),而甜菜碱高中低浓度与癫痫组相比显著降低(P<0.01)。免疫荧光与Western bloting检测GlyR结果,癫痫组GluR的表达与正常对照组相比显著降低(P<0.01),甜菜碱高、中、浓度组较癫痫组显著升高(P<0.01)。结论:甜菜碱具有较好的抗癫痫作用。
Objective: To study the effect of betaine on the expression of glial fibrillary acidic protein (GFAP), glycine (Gly) and glycine receptor (GlyR) in epileptic rats. Methods: Healthy male Wistar rats were randomly divided into normal control group (intraperitoneal injection of normal saline and 1.0 mL saline); epilepsy group (intraperitoneal injection of pentylenetetrazol, 1.0 mL saline); high betaine, Medium and low concentration group (intraperitoneal injection of pentylenetetrazol, betaine gavage); sodium valproate group (intraperitoneal injection of pentylenetetrazol, sodium valproate intragastric administration). The contents of homocysteine in the serum were detected by orbital blood after the experiment; the content of Gly was detected rapidly at low temperature; the level of GFAP was detected by immunofluorescence; GlyR expression changes. Results: There was no significant difference in the latency of major episode in each group (P> 0.05), but the duration of first episode of betaine in treatment group was significantly shorter than that in epilepsy group (P <0.01). The content of homocysteine in epilepsy group was significantly lower than that in the normal group (P <0.01), and the content of homocysteine in high and low concentration of betaine was significantly lower than that in epilepsy group (P <0.05). The content of glycine in epilepsy group was significantly lower than that in normal control group (P <0.01). The contents of glycine in high, middle and low concentration of betaine were significantly higher than those in epilepsy group (P <0.01). Immunofluorescence test GFAP results, epilepsy group was significantly higher than the normal group (P <0.01), while betaine high and low concentrations of epilepsy group was significantly lower (P <0.01). GlyR expression was detected by immunofluorescence and Western blotting. The expression of GluR in epilepsy group was significantly lower than that in the normal control group (P <0.01). The levels of betaine in high, middle and high concentration group were significantly higher than those in epilepsy group (P <0.01). Conclusion: Betaine has better antiepileptic effect.