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目的从鲨鱼肝脏中分离纯化肝细胞再生刺激因子,并研究其分子特点和活性.方法和结果健康鲨鱼肝脏经匀浆、冻融、热提、离心和超滤,在分子量小于3万的组分中检出肝细胞再生刺激因子(HRSF)的活性.此样品经DEAE-Sepharosefast flow柱层析,FPLC Resource 30Q、Resource Q及Mono Q色谱,得到鲨肝细胞再生刺激因子(sHRSF)纯品.SDS-PAGE测定sHRSF的分子量为14 600 Da,紫外扫描显示其特征吸收峰为276 nm,等电点约为5.1,含有18种氨基酸,N端氨基酸序列为LVGPIGAVGPAGKDG.具有显著刺激肝再生生物活性.结论获得了一种未知的新的活性蛋白,即鲨肝细胞再生刺激因子(sHRSF).“,”Aim To purify hepatocyte regeneration stimulatory factor from shark liver and research its molecular feature and activity. Methods and Results Hepatocyte regeneration stimulatory factor (sHRSF) was isolated from healthy shark livers and separated by homogenization, freezing-melting, heat treating, centrifugation, and ultrafiltration. HRSF activity was found mainly in the subfraction of molecular weight less than 30 000 daltons. This crude ultrafiltrate was further purified successively by DEAE-Sepharose fast flow chromatography, FPLC Resource 30Q, Resource Q and Mono Q chromatography. A single band was displayed on sodium dodecyl sulfate polyacrylamide gel electrophoresis, which corresponds to molecular weight of 14 600 daltons. The characteristic absorption was obtained at the wavelength 276 nm. The isoelectric point was about 5.1. It contained 18 amino acids and the 15 N-terminal amino acid residues were LVGPIGAVGPAGKDG. It had a significant activity in stimulating liver to regenerate. Conclusion We obtained an unknown new active protein, that is hep-atocyte regeneration stimulatory factor from shark liver (sHRSF).