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目的 探讨新生儿坏死性小肠结肠炎 (NEC)发病机理 ,为寻找其内源性保护物质提供实验室依据。 方法 以健康 7日龄 Wistar新生鼠分组并制备缺血再灌注 (I/ R)及缺血预处理 (IPC)实验模型 ,分别测定血浆降钙素基因相关肽 (CGRP)浓度、乳酸脱氢酸 (L DH )活性、小肠组织丙二醛(MDA)含量及湿重 /干重 (WW/ DW)比值 ,并制备病理切片光镜下观察形态学变化。 结果 实验各组血浆 CGRP浓度除 IPC组与 IPC+I/ R组差异无显著性外 [(2 92± 17与 2 82± 19) pg/ L(q=1.82 ,P>0 .0 5 ) ],其余各组间差异均有显著性 [(12 4± 10与 16 2± 2 4 ) pg/ L(q=7.16 ) ;(12 4± 10与 2 92± 17)pg/ L(q=4 2 .0 1) ;(16 2± 2 4与 2 92± 17) pg/ L(q=2 1.73,P值均 <0 .0 1) ],IPC组 CGRP较对照组及I/ R组均明显升高。血浆 L DH在 I/ R时较对照组明显升高 [(190± 2 4与 4 6± 9) U / L (q=2 6 .70 ,P<0 .0 1) ],而在 IPC组及 IPC+I/ R组较 I/ R组又有明显下降 [(12 2± 15与 190± 2 4 ) U / L (q=11.77) ;(138± 15与 190± 2 4 ) U/ L(q=6 .39;P值均 <0 .0 1) ]。 I/ R时小肠 MDA含量较对照组明显增加[(1.5 1± 0 .10与 0 .6 1± 0 .0 7) nm ol/ mg(q=36 .12 ,P<0 .0 1) ],而在 IPC及 IPC+I/ R时较 I/ R组则又明显下降
Objective To investigate the pathogenesis of neonatal necrotizing enterocolitis (NEC) and provide a laboratory basis for searching endogenous protective substances. Methods Healthy 7-day-old Wistar newborn rats were divided into four groups: ischemia / reperfusion (I / R) and ischemic preconditioning (IPC). The levels of plasma calcitonin gene related peptide (CGRP), lactate dehydrogenation (L DH) activity, malondialdehyde (MDA) content and wet / dry weight (WW / DW) ratio of small intestine, and the pathological changes were observed under light microscope. Results There was no significant difference in plasma CGRP levels between IPC group and IPC + I / R group ([(92 ± 17 vs2 82 ± 19) pg / L, q = 1.82, P> 0.05) (12 4 ± 10 vs 16 2 ± 2 4) pg / L (q = 7.16); (12 4 ± 10 vs 2 92 ± 17) pg / L (P <0.05). The CGRP in IPC group was significantly higher than that in control group and I / R group Significantly increased. Plasma L DH increased significantly at I / R compared with control group [(190 ± 2 4 vs 46 ± 9) U / L (q = 26.7, P <0.01) (12 ± 15 and 190 ± 2 4) U / L (q = 11.77); (138 ± 15 and 190 ± 24) U / L (q = 6 .39; all P <0. 01)]. Compared with the control group, the content of MDA in small intestine increased significantly at I / R ([(1.51 ± 0.10 vs 0.161 ± 0.07) nm ol / mg (q = 36.12, P <0.01) , While in the IPC and IPC + I / R than the I / R group was significantly decreased