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目前尚未见有绞股蓝对环核苷酸含量影响的报导。本实验观察绞股蓝对小鼠血浆环核苷酸含量的影响。所用广西天等县绞股蓝经蒋承增副教授鉴定,采用水提乙醇沉淀法制备,绞股蓝提取物含生药8克/克。选用19~23克小鼠共26只,雌雄兼取,随机分2组。绞股蓝组用绞股蓝醇提取物按400mg/kg,对照组用等容积凉开水灌胃,每天1次,均灌胃10天。末次给药2小时后快速断头处死,立即取血,作血浆CAMP和cGMP放射免疫测定(按上海中医学院同位素室提供药箱说明书的方法)。
At present, there is no report on the effect of Gynostemma pentaphyllum on the content of cyclic nucleotides. This experiment was to observe the effect of Gynostemma pentaphyllum on plasma cyclic nucleotide content in mice. The Gynostemma used in Guangxi Tiandeng County was identified by Associate Professor Jiang Chengzeng and prepared by water extraction with ethanol precipitation. The Gynostemma pentaphyllum extract contained crude drug 8g/g. A total of 26 males (19-23 g) were selected and divided into 2 groups at random. The Gynostemma group was treated with 400 mg/kg of gynostemma alcohol extract, and the control group was intragastrically administered with equal volume of cold water every day for 10 days. 2 hours after the last administration, the rats were quickly decapitated and blood was taken immediately for plasma CAMP and cGMP radioimmunoassay (methods provided by the Isotope Division of Shanghai Traditional Chinese Medicine Institute).