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随着人类基因组计划的飞速发展,人类遗传密码的解析已临近尾声,数目达10万之巨的基因的功能鉴定将成为迫切需要解决的问题。90年代中期应运而生的生物芯片技术较好地适应了大规模获取和处理生物信息的需要,在生命科学等诸多领域得到了日益广泛的应用,被评为1998年度世界十大科技进展之一。基因芯片是出现最早、技术最成熟、应用最普遍的一种生物芯片,它是利用反向杂交的策略,将DNA 包被在固相支持物的表面,形成高密度 DNA 的微阵列。通过一次杂交就可以同时研究成千上万个基因的表达情况,获得不同组织、细胞以及它们自身之间在不同时期、不
With the rapid development of the human genome project, the analysis of the human genetic code has come to an end, and the functional identification of a huge number of 100,000 genes will become an urgent problem to be solved. The biochip technology that came into being in the mid-1990s is well adapted to the needs of large-scale acquisition and processing of biological information. It has been increasingly used in many fields such as life sciences and was named one of the top ten scientific and technological advancements in the world in 1998. . The gene chip is the earliest, most mature and most commonly used biological chip. It uses a reverse hybridization strategy to coat DNA on the surface of a solid support to form a high-density microarray of DNA. Through a single hybridization, the expression of thousands of genes can be studied simultaneously, and different tissues, cells, and themselves can be obtained at different times, not