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目的 体外观察阿司匹林 (ASA)对胃癌细胞株SGC 790 1细胞增殖的影响 ,并探讨其可能的作用机制。方法 采用噻唑蓝 (MTT)法、流式细胞仪 (FCM )、电镜和3H TdR核素标记等技术 ,研究ASA对SGC 790 1细胞增殖的抑制和可能的机制。结果MTT显示体外ASA对SGC 790 1有细胞毒作用 ,与浓度和作用时间有相关性 ,3H TdR实验表明 ,ASA对细胞DNA合成有抑制作用。FCM显示 ,DNA直方图上出现典型的亚二倍体“凋亡峰” ,凋亡率在 7.8%～ 3 4 .4 %。使S期、G2 /M期细胞比例升高 ,G1期比例下降 ,呈一定剂量效应关系。电镜下见典型的细胞凋亡形态学特征 :细胞核染色质致密浓缩 ,凋亡小体形成等。结论 体外ASA对SGC 790 1细胞增殖有抑制作用 ,可能与诱导细胞凋亡和阻止细胞周期的进展有关。
Objective To observe the effect of aspirin (ASA) on the proliferation of gastric cancer cell line SGC 790 1 in vitro and to explore its possible mechanism. Methods MTT assay, flow cytometry (FCM), electron microscopy and 3H TdR nuclear labeling were used to study the inhibitory effect of ASA on SGC-7901 cell proliferation and its possible mechanism. Results MTT showed that ASA had a cytotoxic effect on SGC 790 1 in vitro, which was related to the concentration and duration of action. The 3H TdR assay showed that ASA inhibited the DNA synthesis. FCM showed a typical sub-diploid “apoptotic peak” on the DNA histogram with a apoptotic rate of 7.8% ~ 34.4%. S phase, G2 / M phase cells increased proportion of G1 phase decreased, showing a dose-response relationship. Under electron microscopy, typical apoptotic morphological features were observed: dense chromatin condensation and formation of apoptotic bodies. Conclusion In vitro ASA can inhibit the proliferation of SGC-7901 cells, which may be related to the induction of apoptosis and the progression of cell cycle arrest.