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血吸虫病是主要累及肝脏的一种疾病。本文使用 DNS—C1荧光反应——聚酰胺薄膜层析法测定晚期血吸虫病(简称晚血)患者和正常人血浆游离氮基酸的组成。然后对每种 DNS—AA 衍生物用 RF—510荧光分光光度计检测,定量分析晚血患者和正常人血浆中11种游离氨基酸的含量,并计算支/芳比值(缬氨酸+亮氨酸+异亮氨酸/苯丙氮酸+酪氨酸)和甘/丙比值(甘氨酸/丙氨酸),以探讨晚血对若干氨基酸代谢的影响。
Schistosomiasis is a disease that mainly affects the liver. In this paper, the composition of plasma free amino acids in patients with advanced schistosomiasis (referred to as late blood) and normal subjects was determined by DNS-C1 fluorescence-polyamide thin film chromatography. Then, each DNS-AA derivative was detected by RF-510 fluorescence spectrophotometer to quantitatively analyze the content of 11 kinds of free amino acids in plasma of patients with late blood and normal persons and calculate the ratio of branch / aromatics (valine + leucine + Isoleucine / phenylalanine + tyrosine) and glycomer / alanine (glycine / alanine) to investigate the effect of late blood on several amino acid metabolism.