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为研究血管形成和血管形成因子表达在肠型胃癌和弥漫型胃癌中的作用，应用抗因子Ⅷ相关抗原抗体、抗ＶＥＧＦ抗体及抗ｂＦＧＦ抗体的免疫组化ＬＳＡＢ法，分别对６３例肠型胃癌和４５例弥漫型胃癌中的微血管数量（ＭＶＣ）、ＶＥＧＦ和ｂＦＧＦ表达进行研究。ＭＶＣ和ＶＥＧＦ及ｂＦＧＦ表达在肠型胃癌明显高于弥漫型胃癌（Ｐ分别＜０．０５，０．００１和０．０１），同样，ＭＶＣ和ＶＥＧＦ表达在肝转移患者明显高于腹膜转移者（Ｐ分别＝０．００１和＜０．０１）；在肠型胃癌中，ＭＶＣ与ＶＥＧＦ表达明显相关（Ｐ＝０．０２），ＭＶＣ和ＶＥＧＦ表达随ＴＮＭ分期增加而增加，而与弥漫型胃癌无关。两型胃癌中的ｂＦＧＦ表达均与ＭＶＣ无关。本研究结果表明肠型胃癌的转移形式为血管依赖性，ＶＥＧＦ可能是诱导肠型胃癌血管形成的一个重要因子。
To study the role of angiogenesis and angiogenic factor expression in intestinal and diffuse gastric cancer, anti-FVIII antibody, anti-VEGF antibody, and anti-bFGF antibody immunohistochemical LSAB method were used for 63 cases of intestinal type gastric cancer, respectively. The expression of microvessels (MVC), VEGF, and bFGF in 45 patients with diffuse gastric cancer were studied. The expression of MVC, VEGF and bFGF in intestinal type gastric cancer was significantly higher than that in diffuse type gastric cancer (P<0.05, 0.001 and 0.01, respectively). Similarly, the expression of MVC and VEGF was significantly higher in patients with liver metastasis than in peritoneal metastasis ( P = 0.001 and <0.01, respectively; in intestinal type gastric cancer, MVC was significantly correlated with VEGF expression (P = 0.02), and MVC and VEGF expression increased with increasing TNM stage, but not with diffuse gastric cancer. . The expression of bFGF in both types of gastric cancer has nothing to do with MVC. The results of this study indicate that the metastatic form of intestinal type gastric cancer is vascular dependence, and VEGF may be an important factor in inducing angiogenesis of intestinal type gastric cancer.