【摘 要】
：
本文通过比较和分析了SSR分子标记技术的三大类DNA提取方法CTAB法、SDS籽粒快速提取法、快速碱煮法,在种子纯度和品种真实性检验中的效果,发现鉴定玉米品种真实性和种子纯度
【机 构】
：
昆明市种子管理站,云南省种子管理站,
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本文通过比较和分析了SSR分子标记技术的三大类DNA提取方法CTAB法、SDS籽粒快速提取法、快速碱煮法,在种子纯度和品种真实性检验中的效果,发现鉴定玉米品种真实性和种子纯度最理想的DNA提取方法是CTAB小量提取DNA法,对于快速检测的理想DNA提取方法是快速碱煮法。
In this paper, by comparing and analyzing the three major categories of DNA extraction methods of SSR molecular markers CTAB method, SDS rapid grain extraction method, rapid alkaline cooking method in the seed purity and variety authenticity test results found that the identification of corn varieties authenticity and The DNA extraction method with the best seed purity is the CTAB DNA extraction method. The ideal DNA extraction method for rapid detection is the rapid alkaline cooking method.
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