论文部分内容阅读
目的从细胞水平观察地塞米松对感染柯萨奇(CoxB_2)病毒的人胚心肌细胞的作用,从而对病毒性心肌炎患者应用激素治疗的问题作出评价。方法采用培养的人胚心肌细胞接种CoxB_2病毒,观察细胞生长、搏动情况及超微结构,测定心肌细胞释放的LDH活性。结果地塞米松治疗组的心肌细胞搏动较感染组者维持时间长,心肌细胞病变发展亦较缓慢,且LDH值明显低于感染组。结论地塞米松对感染CoxB_2病毒的人胚心肌细胞有明显的保护作用,提示临床上重症病毒性心肌炎,特别是早期阶段,应用激素有一定的治疗意义
Objective To observe the effect of dexamethasone on human embryonic cardiomyocytes infected with CoxB_2 at the cellular level to evaluate the application of hormone therapy in patients with viral myocarditis. Methods Cultured human embryonic cardiomyocytes were inoculated with CoxB_2 virus to observe the cell growth, pulsation and ultrastructure. The LDH activity of cardiomyocytes was measured. Results Dexamethasone treatment group myocardial cell beating than the infected group for a long time, the development of myocardial cell disease is also more slowly, and the LDH value was significantly lower than the infected group. Conclusion Dexamethasone has a significant protective effect on human embryonic cardiomyocytes infected with CoxB_2 virus, suggesting that clinical treatment of severe viral myocarditis, especially in the early stage, may have some therapeutic implications