论文部分内容阅读
目的评价温热双蒸水（４５℃ＤＤＷ）＋生理盐水（ＮＳ）＋右旋糖酐－４０（Ｄｅｘｔｒａｎ－４０）序贯腹腔冲洗预防腹腔内肿瘤种植转移的效果。方法将２×１０~６，Ｂ１６－Ｆ１０细胞注入Ｃ５７ＢＬ／６ｊ小鼠腹腔，４ｈｒ后开腹，随机分成 ４组（每组１０只），用下法进行腹腔冲洗：Ａ组（对照组）：ＮＳ＋ＮＳ＋ＮＳ；Ｂ组：４５℃ＤＤＷ＋ＮＳ＋Ｄｅｘｔｒａｎ－４０；Ｃ组：４５℃ ＤＤＰ＋ＮＳ＋ＮＳ；Ｄ组：４５℃ ＤＤＷ＋ＮＳ＋Ｄｅｘｔｒａｎ－４０＋ＤＤＰ。３周后处死小鼠行病检。结果 Ａ组 １００％致瘤，Ｄ、Ｂ、Ｃ组致瘤鼠数分别为１只，３只，３只，与Ａ组比较均差异显著（ Ｐ＝０．０００１，Ｐ＝０． ００３１， Ｐ＝０． ００３１）。但 Ｃ、Ｄ两组各有１只鼠因ＤＤＰ毒性死于肾囊。结论运用本研究的动物模型，４５℃ＤＤＷ＋ＮＳ＋Ｄｅｘ－ｔｒａｎ－４０序贯腹腔冲洗可预防７０％的Ｃ５７ＢＬ／６ｊ小鼠发生腹膜癌，且未发现有任何毒副作用；在此基础上；加用 ＤＤＰ，疗效可增至 ８８．９％，但对小鼠的毒性也随之增加。
Objective To evaluate the effect of sequential intraperitoneal irrigation on prevention of intraperitoneal tumor implantation in warm double distilled water (45 ℃ DDW) + normal saline (NS) + dextran-40 (Dextran-40). Methods C57BL / 6j mice were injected intraperitoneally with 2 × 10 ~ 6 cells and B16-F10 cells respectively. After 4 hours, they were divided into 4 groups randomly (10 in each group) NS + NS + NS; Group B: DDW + NS + Dextran-40 at 45 ° C; Group D: DDP + NS + NS at 45 ° C; Group D: DDW + NS + Dextran-40 + DDP at 45 ° C. Three weeks later the mice were sacrificed. Results A group of tumorigenicity of 100%, D, B, C group of tumor-causing rats were 1, 3, 3, compared with the A group were significantly different (P = 0.0001, P = 0.0031, P = 0. 0031). However, one of the two groups C and D died of renal toxicity due to DDP. CONCLUSIONS: Peritoneal washing with DDW + NS + Dex-tran-40 at 45 ° C prevented peritoneal carcinogenesis in 70% of C57BL / 6j mice and no toxic or side effects were found using the animal model of this study. On the basis of this study, DDP, Efficacy can be increased to 88.9%, but the toxicity of mice also increased.