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目的评价温热双蒸水(45℃DDW)+生理盐水(NS)+右旋糖酐-40(Dextran-40)序贯腹腔冲洗预防腹腔内肿瘤种植转移的效果。方法将2×10~6,B16-F10细胞注入C57BL/6j小鼠腹腔,4hr后开腹,随机分成 4组(每组10只),用下法进行腹腔冲洗:A组(对照组):NS+NS+NS;B组:45℃DDW+NS+Dextran-40;C组:45℃ DDP+NS+NS;D组:45℃ DDW+NS+Dextran-40+DDP。3周后处死小鼠行病检。结果 A组 100%致瘤,D、B、C组致瘤鼠数分别为1只,3只,3只,与A组比较均差异显著( P=0.0001,P=0. 0031, P=0. 0031)。但 C、D两组各有1只鼠因DDP毒性死于肾囊。结论运用本研究的动物模型,45℃DDW+NS+Dex-tran-40序贯腹腔冲洗可预防70%的C57BL/6j小鼠发生腹膜癌,且未发现有任何毒副作用;在此基础上;加用 DDP,疗效可增至 88.9%,但对小鼠的毒性也随之增加。
Objective To evaluate the effect of sequential intraperitoneal irrigation on prevention of intraperitoneal tumor implantation in warm double distilled water (45 ℃ DDW) + normal saline (NS) + dextran-40 (Dextran-40). Methods C57BL / 6j mice were injected intraperitoneally with 2 × 10 ~ 6 cells and B16-F10 cells respectively. After 4 hours, they were divided into 4 groups randomly (10 in each group) NS + NS + NS; Group B: DDW + NS + Dextran-40 at 45 ° C; Group D: DDP + NS + NS at 45 ° C; Group D: DDW + NS + Dextran-40 + DDP at 45 ° C. Three weeks later the mice were sacrificed. Results A group of tumorigenicity of 100%, D, B, C group of tumor-causing rats were 1, 3, 3, compared with the A group were significantly different (P = 0.0001, P = 0.0031, P = 0. 0031). However, one of the two groups C and D died of renal toxicity due to DDP. CONCLUSIONS: Peritoneal washing with DDW + NS + Dex-tran-40 at 45 ° C prevented peritoneal carcinogenesis in 70% of C57BL / 6j mice and no toxic or side effects were found using the animal model of this study. On the basis of this study, DDP, Efficacy can be increased to 88.9%, but the toxicity of mice also increased.