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利用室内选育119代的抗杀虫双、抗杀螟丹小菜蛾品系和敏感品系,研究了各杂交世代小菜蛾对杀虫双和杀螟丹的敏感性.通过反复回交建立小菜蛾对杀虫双和杀螟丹抗性的近等基因系.测定了各杂交世代和近等基因系的多功能氧化酶环氧化活性.结果表明,抗性品系和抗性近等基因系多功能氧化酶环氧化活性比敏感品系高,杀虫双、杀螟丹抗性品系和杀虫双、杀螟丹抗性近等基因系分别是敏感品系的1.62、1.83、1.56和1.97倍,因此,多功能氧化酶环氧化活性提高与小菜蛾对杀虫双和杀螟丹抗性有关
The resistance to insecticidal double and the cartap in diamondback moth (Plutella xylostella) at each cross was studied using 119 generations of anti-insecticidal double-resistant and resistant to diamondback moth (Plutella xylostella) and sensitive strains. Near-isogenic lines resistant to insecticidal diphosphate and cartap resistance to Plutella xylostella were established by repeated backcrossing. The epoxidation activities of multifunctional oxidases of each cross and near isogenic lines were determined. The results showed that the epoxidation activities of the multifunctional oxidase in the resistant and near isogenic lines were higher than those in the susceptible lines, and the resistance to insecticides, cartap resistance and insecticidal double and cartap resistance genes Were 1.62, 1.83, 1.56 and 1.97 times of the susceptible lines, respectively. Therefore, the increase of polyoxygenase epoxidation activity was related to the resistance of diamondback moth to insecticidal double and cartap.