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目的探讨刺五加注射液对人肺腺癌A549细胞的体外增殖的抑制作用。方法采用MTT法测定了不同浓度的刺五加注射液对人肺腺癌A549细胞的体外抑制作用,倒置显微镜观察细胞形态变化。结果刺五加注射液浓度越高对A549细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论刺五加注射液对人肺腺癌A549细胞的增殖有较强的抑制作用。
Objective To investigate the inhibitory effect of acanthopanax senticosus injection on the proliferation of human lung adenocarcinoma A549 cells in vitro. Methods MTT assay was used to determine the inhibitory effect of acanthopanax senticosus injection on human lung adenocarcinoma A549 cells in vitro. The morphological changes of cells were observed by inverted microscope. Results The higher the concentration of Acanthopanax senticosus injection, the better the inhibitory effect on A549 cells. The longer the effect was, the stronger the inhibition effect was. The relationship between dose-effect and time-effect was good. Morphological observation showed apoptosis. Conclusion Acanthopanax senticosus injection has a strong inhibitory effect on the proliferation of human lung adenocarcinoma A549 cells.