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以WI998为母本(纯雌株、厚皮网纹甜瓜品系),TopMark为父本(雄全同株纯合品系),配置杂交组合,利用单粒传得到171个株系的重组自交系(F2S4)群体构建甜瓜SSR分子标记遗传连锁图谱。该连锁图谱包含19个连锁群,覆盖基因组长度为1414.2cM,标记间平均距离为10.2cM。对结实花初花节位开展QTL分析,共检测到9个QTL,分别分布在第1、9、10、11连锁群上,各QTL的LOD值在2.89~9.42之间,4个QTL贡献率超过10%。位于第9连锁群的QTLFp9.4贡献率最大,为18.57%(2010秋季LOD=9.17);位于第9连锁群的Fp9.3(2010春季8.64%,LOD=6.44;2010秋季17.99%,LOD=9.42),位于第10连锁群的Fp10.1(2010春季3.59%,LOD=2.89;2010秋季6.20%,LOD=3.43)在两季的位置都很稳定。获得与结实花初花节位紧密连锁(<10cM)的8个特异标记(SSR01737、MU141991、TJ105、GCM206、SSR04910、MU173563、NR52、MU146331),为进一步开展QTL精细定位提供参考。
WI998 was used as the female parent (pure female and muskmelon), and TopMark was used as the male parent (homozygous homozygotes of male and female), and the hybrid combinations were constructed. The recombinant inbred lines (F2S4) population to construct melon SSR molecular marker genetic linkage map. The linkage map contains 19 linkage groups, covering the genome length of 1414.2cM, the average distance between markers was 10.2cM. A total of 9 QTLs were detected in QTLs for early flowering stages of Fagaceae, which were distributed on the 1st, 9th, 10th and 11th linkage groups respectively. The LOD values of QTLs ranged from 2.89 to 9.42, and the four QTLs Over 10%. The QTLFp9.4 in the 9th linkage group had the highest contribution rate of 18.57% (LOD = 9.17 in the autumn of 2010), Fp9.3 (8.64% in spring 2010, LOD = 6.44 in the 2010 linkage group, 17.99% in the autumn of 2010, LOD = 9.42). Fp10.1 (3.59% in spring 2010, LOD = 2.89; 6.20% in autumn 2010, LOD = 3.43) located in the 10th linkage group was stable in both seasons. Eight SSR markers (SSR01737, MU141991, TJ105, GCM206, SSR04910, MU173563, NR52, MU146331) closely linked to the flower primrose locus were obtained, which could provide reference for further fine mapping of QTLs.