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目的探讨高功率微波导致卵巢组织形态学改变的规律及其特点。方法雌性Wistar大鼠60只,随机分为对照组(C)和照射组(R),照射组大鼠经平均功率密度100 m W/cm2的S-波段HPM辐照15 min,分别于辐照后1 d、3 d、7 d、14 d、28 d和3 m活杀,取双侧卵巢,制作石蜡切片和超薄切片,光镜和电镜下观察组织形态学改变,并分别对卵泡和黄体数量进行定量计数。结果照射后卵巢组织结构发生明显损伤:原始闭锁卵泡和囊状闭锁卵泡数量持续性增多(P<0.05),囊状卵泡存活率显著降低(P<0.05);黄体数量进行性增多(P<0.05),颗粒黄体细胞凋亡增多;其中,囊状卵泡、颗粒细胞和颗粒黄体细胞损伤尤为严重。结论高功率微波可导致大鼠卵巢组织形态学改变,具有广泛性、速发性、持续性和不同卵泡与细胞敏感程度差异性特点。
Objective To investigate the regularity and characteristics of ovarian tissue morphological changes induced by high power microwave. Methods Sixty female Wistar rats were randomly divided into control group (C) and irradiation group (R). The irradiated rats were irradiated with S-band HPM with an average power density of 100 mW / cm2 for 15 min. After 1 d, 3 d, 7 d, 14 d, 28 d and 3 m live kill, bilateral ovaries were taken to make paraffin sections and ultrathin sections. Morphological changes were observed under light microscope and electron microscope. Quantities of corpus luteum count. Results The structure of ovarian tissue was obviously damaged after irradiation: the number of primordial atresia follicles and cystic atresia follicles continued to increase (P <0.05), the survival rate of cystic follicles was significantly decreased (P <0.05); the number of corpus luteum increased progressively (P <0.05 ), Increased apoptosis of granular luteal cells; Among them, the cystic follicles, granulosa cells and granular luteal cells damage is particularly serious. Conclusion High-power microwave can induce the morphological changes of ovarian tissue in rats, with the features of broadness, rapid onset, persistence and different sensitivity of follicles and cells.