To present the clinical application of the multispectral imaging colposcopic s ystem (MIS colposcopy). MIS colposcopy was performed on 123 enrolled women. Afte r a 3%acetic acid application, sequential images were captured, analyzed, and s tored automatically. Directed biopsies were taken from distinct marked acetic ac id-responsive tissue areas indicated on the monitor, while a real-time assessm ent of the curves of intensity of the backscattered light (IBSL) vs. time was pe rformed. Blind biopsies were taken from non-acetowhitening areas. Histological findings were correlated with MIS colposcopy results and compared with conventio nal colposcopy and Pap test results. Acetic acid-tissue interaction resulted in temporal and spatial alterations to the light scattering properties of the abno rmal tissue that was analyzed. The shape of IBSL curve and the “relaxation time” (the time it takes for IBSL to decay to 1/e of its peak value) changed in accor dance with the underlying lesion. More severe CIN lesions lead to higher maximum IBSL; longer durations of acetowhitening lead to increasingly delayed exponenti al decay of IBSL curve. To compare with histological examination, MIS colposcopy had a 1.7%falsediagnostic rate, while PAP test and conventional colposcopy had 24.4%and 22%false-diagnostic rates, respectively. A triple exponential funct ion created a “pseudocolor“ image that comprised the grade map of the lesion, a nd this is frequently representative of the duration/degree of the induced alter ations. Improved diagnostic information can be gained by recording the optical i nformation in a narrow spectral range with high spatial resolution. MIS colposco py can be used in the diagnosis of uterine cervix pathological conditions and in the differentiation between CIN lesions. To present the clinical application of the multispectral imaging colposcope s ystem (MIS colposcopy). MIS colposcopy was performed on 123 enrolled women. Afte ra 3% acetic acid application, sequential images were captured, analyzed, and s tored automatically. from distinct marked acetic acid-responsive tissue areas indicated on the monitor, while a real-time assessm ent of the curves of intensity of the backscattered light (IBSL) vs. time was pe rformed. Blind biopsies were taken from non-acetowhitening areas Histological findings were correlated with MIS colposcopy results and compared with conventio nal colposcopy and Pap test results. Acetic acid-tissue interaction resulted in temporal and spatial alterations to the light scattering properties of the abno rmal tissue that was analyzed. The shape of IBSL curve and the ”relaxation time “ (the time it takes for IBSL to decay to 1 / e of its peak value) changed in accor dance with the underlying lesion. Mo re severe CIN lesions lead to higher maximum IBSL; longer durations of acetowhitening lead to increasingly delayed exponentiation al decay of IBSL curve. To compare with histological examination, MIS colposcopy had a 1.7% falsed diagnostic rate, while PAP test and conventional colposcopy had 24.4% and 22% false-diagnostic rates, respectively. A triple exponential funct ion created a ”pseudocolor" image that comprised the grade map of the lesion, a nd this is frequent representative of the duration / degree of the induced alter ations. Improved diagnostic information can be acquired by recording the optical i nformation in a narrow spectral range with high spatial resolution. MIS colposco py can be used in the diagnosis of uterine cervix pathological conditions and in the differentiation between CIN lesions.