论文部分内容阅读
探讨雷公藤内酯醇 (TP )对致敏小鼠T淋巴细胞IL 5mRNA表达的影响及其机制。采用卵蛋白 (OVA )致敏的方法建立模型 ;运用原位杂交染色法 (ISH )观察TP对T淋巴细胞IL 5mRNA表达的影响 ;通过凝胶电泳迁移率实验 (EMSA )对CD4+T淋巴细胞核转录因子GATA 3的DNA结合活性进行检测 ,同时就TP的作用与地塞米松 (DM )相比较。结果表明致敏小鼠T淋巴细胞IL 5mRNA表达显著高于正常对照组 (P <0 0 1) ,经TP、DM处理后 ,其IL 5mRNA表达显著低于致敏组(P <0 0 1)。致敏小鼠CD4+ T淋巴细胞体外经伴刀豆蛋白A (ConA )刺激后 ,GATA 3的DNA结合活性与正常对照组比较显著增强 ,并呈时间依赖关系 ,经TP、DM处理后 ,GATA 3的DNA结合活性显著减弱。TP抑制IL 5基因转录的分子机制可能与其抑制GATA 3的DNA结合活性有关。
To investigate the effects of triptolide (TP) on IL-5 mRNA expression in T lymphocytes of sensitized mice and its mechanism. The effect of TP on the expression of IL-5 mRNA in T lymphocytes was observed by in situ hybridization staining (ISH). The effects of TP on the expression of IL-5 mRNA in T lymphocytes were evaluated by electrophoretic mobility shift assay (EMSA) The DNA binding activity of the transcription factor GATA 3 was tested, while the effect of TP was compared with that of dexamethasone (DM). The results showed that the expression of IL-5 mRNA in T lymphocytes of sensitized mice was significantly higher than that of the normal control group (P <0.01). After TP and DM treatment, the expression of IL-5 mRNA was significantly lower than that of sensitized mice (P <0.01) . GATA-3 DNA-binding activity of sensitized mice CD4 + T lymphocytes stimulated with concanavalin A (ConA) in vitro was significantly increased compared with that of the normal control group in a time-dependent manner. After TP and DM treatment, GATA 3 DNA binding activity was significantly weakened. The molecular mechanism by which TP inhibits the transcription of IL-5 may be related to its inhibition of DNA-binding activity of GATA3.