腺嘌呤二磷酸葡萄糖酸化酶 (GLgcTM)是植物淀粉生物合成过程中一个起关键性调节作用的酶。应用农杆菌介导法 ,将外源GLgcTM基因导入粳稻软米愈伤组织中 ,通过愈伤组织的预培养 ,农杆菌的预培养 ,酚类化合物的使用及抗生素种类、浓度的选择等方法 ,有效地提高了抗性愈伤组织的形成率及再分化率 ,并获得了转基因植株。经PCR检测 ,初步证实已将目的基因整合到受体植物的基因组中。 Adenine diphosphate glucoamylase (GLgcTM) is a key regulator of plant starch biosynthesis. The Agrobacterium-mediated method was used to introduce the exogenous GLgcTM gene into the callus of Japonica rice. Through pre-culturing of callus, pre-culture of Agrobacterium, the use of phenolic compounds and the selection of antibiotic type and concentration, The effective rate of callus formation and re-differentiation was increased and transgenic plants were obtained. PCR test confirmed that the target gene has been integrated into the genome of the recipient plant.