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为探讨甘露糖及其受体在小鼠精子 -透明带结合中的作用 ,小鼠附睾尾精子经 M1 6工作液获能培养 2 h后 ,分别用 DMA(甘露糖基化的牛血清白蛋白 )、D-甘露糖处理1 5 min,然后与未作处理的带透明带卵子共培养 3 0 min;另 2组卵子分别用甘露糖苷酶、Con A(植物凝集素 )处理 1 5 min后分别与未作处理的获能精子共培养 3 0 min.卵子固定 ,在相差显微镜下计数各组卵子的精子结合数.结果表明 :DMA、D-甘露糖、甘露糖苷酶、Con A对精子和透明带结合有抑制作用.提示甘露糖及其相应受体在精卵相互作用中可能具有调节作用“,”To investigate the role of mannose and mannose receptor in sperm zona binding in mouse. Capacitated spermatozoa, preincubated in the presence of D mannose(DM) or D mannosylated albumin(DMA), were co incubated with zona onclosed eggs pretreated with α Mannosidase or Concanavalin A (ConA) respectively at the same time. The role of DM 、DMA、α Mannosidase、ConA in the interaction between mouse sperm and zona were studied by testing their effects on binding sperm to eggs. The results showed that the DM 、DMA、α Mannosidase、ConA decrease the number of sperm bound per zona pellucida. This findings suggested that mannose receptor may have a role in the interaction between sperm zona.