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目的了解云南省啮齿动物及人血清中携带SARS冠状病毒(SARS-CoV)抗体状况。方法在居民区用笼夜法捕鼠并采集鼠血清,在当地采集健康人群血清。用ELISA双抗原夹心法和间接ELISA法分别检测鼠和人血清中SARS-CoV IgG抗体。结果 2003-2004年在云南省16个县(区、市)捕获啮齿动物3属6种807只,黄胸鼠、褐家鼠、大足鼠、大绒鼠、小家鼠和斯氏家鼠构成比依次为46.22%、38.04%、8.67%、4.71%、1.98%和0.37%。动物血清SARS-CoV IgG抗体阳性率为0.62%(5/813),其中黄胸鼠阳性3份、褐家鼠阳性2份。健康人血清SARSCoV抗体阳性率为0.73%(4/548)。结论云南省啮齿动物和健康人群中极有可能存在SARS-CoV感染,应加强云南省啮齿动物及其它野生动物SARS-CoV的监测。
Objective To understand the status of antibodies against SARS coronavirus (SARS-CoV) in rodents and human serum of Yunnan province. Methods In the residential area with cage night catching rat and collecting rat serum, healthy people in the local collection of serum. The SARS-CoV IgG antibodies in murine and human sera were separately detected by ELISA double antigen sandwich method and indirect ELISA method. Results 807 rodents, 3 genera and 6 species were captured in 16 counties (districts and cities) of Yunnan Province from 2003 to 2004. The composition ratio of Rattus flavipectus, Rattus norvegicus, Dazu rat, Followed by 46.22%, 38.04%, 8.67%, 4.71%, 1.98% and 0.37% respectively. The positive rate of SARS-CoV IgG in animal serum was 0.62% (5/813), of which 3 were Rattus norvegicus and 2 Rattus norvegicus. The positive rate of serum SARSCoV antibody in healthy volunteers was 0.73% (4/548). Conclusion SARS-CoV infection is most likely to occur in rodents and healthy people in Yunnan Province. SARS-CoV surveillance in rodents and other wildlife in Yunnan should be strengthened.