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目的了解临床分离的43株鲍曼不动杆菌中Ⅰ类、Ⅱ类、Ⅲ类整合子的流行病学现状及其耐药性。方法用多重PCR方法扩增30株鲍曼不动杆菌Ⅰ类、Ⅱ类、Ⅲ类整合酶基因,用K-B法检测鲍曼不动杆菌的耐药情况。结果Ⅰ类整合子检出率为79.1%(34/43),未检出Ⅱ、Ⅲ类整合子。结论Ⅰ类整合子阳性的菌株的耐药率较高,显著高于Ⅰ类整合子阴性的菌株,多重PCR是筛查革兰阴性杆菌整合子的有效方法。
Objective To understand the epidemiological status and drug resistance of class Ⅰ, class Ⅱ and class Ⅲ integrons in 43 strains of Acinetobacter baumannii isolated clinically. Methods Multiplex PCR amplification of Acinetobacter baumannii class Ⅰ, Ⅱ and Ⅲ integrase gene, K-B method to detect Acinetobacter baumannii drug resistance. Results The detection rate of class Ⅰ integron was 79.1% (34/43). No class Ⅱ or Ⅲ integron was detected. Conclusions The positive rate of integron Ⅰ integron is higher than that of the negative class Ⅰ integron. Multiplex PCR is an effective method to screen Gram - negative bacilli integron.